6,213 research outputs found
Becoming the Gothic Archive: From Digital Collection to Digital Humanities
The Gothic Archive is the flagship digital humanities project for the Marquette University library. The project was birthed from a simple digital collection, and through the partnership of faculty and librarians, was transformed into something more. The core tenets of digital collection creation were adhered to in order to create a solid foundation upon which to build the Archive. The expertise of both groups and communication were key in the evolution of the collection, and in discovering and highlighting the relationships between the objects. This case study reviews the steps Marquette took in creating the collection and taking it to the level of digital humanities project
Climate change mitigation by Greater Adelaide councils
This paper reports on carbon mitigation actions adopted by Greater Adelaide councils (n=14) in South Australia. A survey of environmental officers profiled carbon mitigation actions, emissions auditing, and motives for emissions reduction by councils. The main reasons for carbon actions were a climate change plan, showing climate leadership, and cost savings
The Turk and the Yankee: A Cross-Cultural Comparison between Turkish and American Managers
With Turkeyâs developing role as a lead nation among emerging markets, the field of cross-cultural management becomes a key contributor to the interactions between Turkish and American professionals in the workplace. This study uses models of national cultural differences based primarily on the findings of Hofstede (1984), but it also incorporates those of Trompenaars and Hampden-Turner (1998) and the GLOBE project (2002), resulting in a comparative cross-cultural management analysis. In combining both academic theory and actual international experience, this paper illustrates that, through effective cross-cultural management, profound understanding and harmony can exist between international managers
Regulation of the hrp Type III Secretion System in Pseudomonas syringae pv. tomato DC3000
Pseudomonas syringae pv. DC3000 is a gram-negative bacterium that infects the model
plant Arabidopsis thaliana. Pathogenicity is achieved via secretion of effector proteins into
the host cytoplasm through a Type III Secretion System (T3SS). In Ps. DC3000 the T3SS (and
associated effector proteins) are dependent on HrpL for their transcription. hrpL
transcription is sigma54-dependent and requires two co-dependent enhancer binding
proteins, HrpR and HrpS (HrpRS), for activation. HrpRS are regulated by two hrpL-dependent
proteins, HrpV and HrpG, where HrpV negatively affects HrpRS activity and HrpG relieves
this repression.
Here the mechanism of HrpV and HrpGâs action on HrpRS activity was tested in vivo and in
vitro; and the molecular determinants of HrpV and HrpG functionality were characterised by
in silico and mutational analysis. Whole-gene deletion mutants of hrpV and hrpG in Ps.
DC3000 revealed complications associated with inserting marker cassettes in
transcriptionally-antagonistic orientations. Truncation mutants of HrpV and HrpG showed
that C-terminal helices in both proteins play a functional and/or structural role. Alanine
mutants indicated a structural role for residues 108-110 in HrpV and a functional role for
residues 111-113 in HrpG (NQR motif). HrpV was demonstrated to form a dimer and
represses the activity of HrpRS (in vivo and in vitro) but not via inhibiting ATPase activity or
destabilising open promoter complexes. HrpG was shown to destabilise the HrpV dimer
(potentially via the C-terminal NQR motif). Findings from this study were used to propose a
revised model of hrpL regulation in which an active dimer of HrpV binds two adjacent HrpS
subunits of a HrpRS hexamer to âlockâ the hexamer in an unfavourable conformation. HrpG
acts by destabilising the HrpV dimer into an inactive monomeric form. Additional
experiments to confirm this model include creating an artificially linked dimer of HrpV and
testing its repressive action on HrpRS in the presence or absence of HrpG
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